Composite

Part:BBa_K1423003:Design

Designed by: Shawna Henry   Group: iGEM14_WPI-Worcester   (2014-09-23)


BCLA-YFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When cloning, we had to ensure that the YFP would stay in frame after the scar site in the BCLA.


Source

The BCLA is from bacillus antracis and the YFP is a mutant form of GFP from the jellyfish aequorea victoria.

References